Journal: World Journal of Gastroenterology

Article Title: Role of gut microbiota in Crohn’s disease pathogenesis: Insights from fecal microbiota transplantation in mouse model

doi: 10.3748/wjg.v30.i31.3689

Figure Lengend Snippet: Histopathological alterations in mesenteric adipose and intestinal tissues of patients with Crohn’s disease. A: Mesenteric adipose tissues from Crohn’s disease (CD) patients and non-CD subjects stained with hematoxylin-eosin to assess histopathological alterations (× 150); B: Real-time reverse transcriptase-polymerase chain reaction assays of the levels of expression of interleukin-6 ( IL-6 ), IL-1β , tumour necrosis factor alpha , MCP-1 , leptin , and adiponectin mRNAs; C: Intestinal tissues from CD patients and non-CD subjects stained with Masson stain to evaluate fibrotic alterations (× 150); D: Immunohistochemical staining of alpha-smooth muscle actin in intestinal tissues from CD patients and non-CD subjects (× 150). Average optical density was quantified using ImageJ software (right panel). n = 12, b P < 0.01 or n = 3, b P < 0.01 by paired t -tests. HE: Hematoxylin and eosin; TNBS: 2,4,6-trinitrobenzene sulfonic acid; CD: Crohn’s disease; TNF-α: Tumour necrosis factor alpha; IL: Interleukin; α-SMA: Alpha-smooth muscle actin; AOD: Average optical density.

Article Snippet: A 100 μL aliquot of serum was added to each well of an ELISA plate included in ELISA kits measuring serum concentrations of endotoxin (CSB-E13066m, CUSABIO, Wuhan, Hubei Province, China), IL-6 (CSB-E04639m, CUSABIO), IL-1β (Ab197742, Abcam, Cambridge, United Kingdom), TNF-α (CSB-E04741m, CUSABIO), MCP-1 (Ab208979, Abcam), leptin (ab100718, Abcam), and adiponectin (CSB-E07272m, CUSABIO).

Techniques: Staining, Reverse Transcription, Polymerase Chain Reaction, Expressing, Immunohistochemical staining, Software

Journal: World Journal of Gastroenterology

Article Title: Role of gut microbiota in Crohn’s disease pathogenesis: Insights from fecal microbiota transplantation in mouse model

doi: 10.3748/wjg.v30.i31.3689

Figure Lengend Snippet: ELISA determinations of serum concentrations of cytokines and adipokines. A: Interleukin (IL)-6; B: IL-1β; C: Tumour necrosis factor alpha; D: MCP-1; E: Leptin; F: Adiponectin. n = 6, b P < 0.01 vs control; a P < 0.05, b P < 0.01 vs 2,4,6-trinitrobenzene sulfonic acid (TNBS) alone; b P < 0.01 vs TNBS + NC-fetal microbiota transplantation, by one-way ANOVA with post-hoc Tukey HSD test. TNBS: 2,4,6-trinitrobenzene sulfonic acid; CD: Crohn’s disease; NC: Normal control; FMT: Fetal microbiota transplantation.

Article Snippet: A 100 μL aliquot of serum was added to each well of an ELISA plate included in ELISA kits measuring serum concentrations of endotoxin (CSB-E13066m, CUSABIO, Wuhan, Hubei Province, China), IL-6 (CSB-E04639m, CUSABIO), IL-1β (Ab197742, Abcam, Cambridge, United Kingdom), TNF-α (CSB-E04741m, CUSABIO), MCP-1 (Ab208979, Abcam), leptin (ab100718, Abcam), and adiponectin (CSB-E07272m, CUSABIO).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Transplantation Assay

Journal: World Journal of Gastroenterology

Article Title: Role of gut microbiota in Crohn’s disease pathogenesis: Insights from fecal microbiota transplantation in mouse model

doi: 10.3748/wjg.v30.i31.3689

Figure Lengend Snippet: Effects of gut microbiota from Crohn’s disease patients on the histopathological characteristics of mesenteric adipose and intestinal tissues from mice with 2,4,6-trinitrobenzene sulfonic acid-induced Crohn’s disease. A: Mesenteric adipose tissues collected from mice were stained with hematoxylin-eosin to assess histopathological alterations (× 150); B and C: Real-time reverse transcriptase-polymerase chain reaction assays of interleukin-6 ( IL-6 ), IL-1β , tumour necrosis factor alpha , MCP-1 , leptin , adiponectin , LXR , and FXR mRNA levels in mouse tissues; D: Intestinal tissues from mice stained with Masson stain to evaluate fibrotic alterations (× 150); E: Immunoblotting assays of alpha-smooth muscle actin and vimentin protein levels in mouse intestinal tissues. n = 6 or 3, b P < 0.01 vs control; a P < 0.05, b P < 0.01 vs 2,4,6-trinitrobenzene sulfonic acid (TNBS) alone; b P < 0.01 vs TNBS + NC-fetal microbiota transplantation, by one-way ANOVA with post-hoc Tukey HSD test. TNBS: 2,4,6-trinitrobenzene sulfonic acid; CD: Crohn’s disease; NC: Normal control; FMT: Fetal microbiota transplantation; IFN-γ: Interferon-gamma; TNF-α: Tumour necrosis factor alpha; IL: Interleukin; HE: Hematoxylin and eosin; α-SMA: Alpha-smooth muscle actin.

Article Snippet: A 100 μL aliquot of serum was added to each well of an ELISA plate included in ELISA kits measuring serum concentrations of endotoxin (CSB-E13066m, CUSABIO, Wuhan, Hubei Province, China), IL-6 (CSB-E04639m, CUSABIO), IL-1β (Ab197742, Abcam, Cambridge, United Kingdom), TNF-α (CSB-E04741m, CUSABIO), MCP-1 (Ab208979, Abcam), leptin (ab100718, Abcam), and adiponectin (CSB-E07272m, CUSABIO).

Techniques: Staining, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Control, Transplantation Assay

Journal: Nutrients

Article Title: The Effect of Diet on Improved Endurance in Male C57BL/6 Mice

doi: 10.3390/nu10081101

Figure Lengend Snippet: The effect of GF diets on the exercise signaling pathways and autophagy. The expression of GF/exercise-associated adiponectin levels were evaluated by ELISA. Mice that were fed control or GF diets were evaluated for adiponectin levels. The results are expressed as mean ± SD ( n = 10 per group), * p < 0.01 compared to the control group.

Article Snippet: Plasma adiponectin levels were determined by ELISA kits (MRP300, R&D Systems, Minneapolis, MN, USA).

Techniques: Protein-Protein interactions, Expressing, Enzyme-linked Immunosorbent Assay, Control